1bit

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THE CRYSTAL STRUCTURE OF ANIONIC SALMON TRYPSIN IN A SECOND CRYSTAL FORM

Overview

Anionic salmon trypsin in a second crystal form (ST-IIB) has been refined, at 1.83 A, resolution. The crystals are orthorhombic and belong to space, group P2(1)2(1)2 with lattice parameters a = 77.09, b = 82.33 and c =, 31.16 A. The present structure has been compared to salmon trypsin as it, appears in a previously reported crystal form (ST-IIA) with cell, dimensions a = 61.95, b = 84.33 and c = 39.11 A [Smalas & Hordvik (1993)., Acta Cryst. D49, 318-330]. The presence of a sulfate group involved in, several hydrogen bonds to active-site residues, and the location of an, additional benzamidine site in the crystal lattice, are the most striking, differences between the present and the previous structure. Superposition, of main-chain atoms in the two structures give an overall r.m.s., difference of 0.26 A, with the main differences located to areas with, different molecular packing. The overall coordinate error is estimated to, be between 0.20 and 0.25 A, by the method of Luzzati.

About this Structure

1BIT is a Single protein structure of sequence from [1] with CA, SO4 and BEN as ligands. Full crystallographic information is available from OCA.

Reference

Structure of anionic salmon trypsin in a second crystal form., Berglund GI, Smalas AO, Hordvik A, Willassen NP, Acta Crystallogr D Biol Crystallogr. 1995 Sep 1;51(Pt 5):725-30. PMID:15299802

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