1bm4
From Proteopedia
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MOMLV CAPSID PROTEIN MAJOR HOMOLOGY REGION PEPTIDE ANALOG
Overview
The capsid domain of retroviral Gag proteins possesses a single highly, conserved subdomain termed the major homology region (MHR). While the, mutagenesis of residues in the MHR will impair virus infectivity, the, precise solution structure and function of the MHR is not known. To aid, the structure/function characterization of the MHR, the structures of, synthetic peptides encompassing the MHR of the human immunodeficiency, virus type I (HIV-1) and Moloney murine leukemia virus (MoMLV) capsid, proteins were investigated by several techniques. Homology-based, secondary-structure prediction suggested that the HIV-1 and MoMLV peptides, could form 50% and 38% alpha-helix, respectively. CD studies indicated, that, in the presence of 50% trifluoroethanol, the HIV-1 peptide adopts an, alpha-helical structure over half of its length, while the MoMLV peptide, is over one third alpha-helix. Further analysis by 1H-NMR suggested that, the C-terminal portion of the MHR of each virus forms a helix in aqueous, solution. Distance-geometry structures of each peptide were calculated, from NOE distance restraints and were refined by restrained molecular, dynamics. The C-terminal halves of both peptides were observed to be in an, alpha-helical conformation, while the N-terminal halves were disordered., Furthermore, both helices were amphipathic with high conservation of amino, acid side-chain character, suggesting that a conserved helical MHR, C-terminus is essential to retroviral capsid protein function.
About this Structure
1BM4 is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Solution structures of human immunodeficiency virus type 1 (HIV-1) and moloney murine leukemia virus (MoMLV) capsid protein major-homology-region peptide analogs by NMR spectroscopy., Clish CB, Peyton DH, Barklis E, Eur J Biochem. 1998 Oct 1;257(1):69-77. PMID:9799104
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