1bp7

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Image:1bp7.gif

1bp7, resolution 3.00Å

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GROUP I MOBILE INTRON ENDONUCLEASE I-CREI COMPLEXED WITH HOMING SITE DNA

Overview

The structure of the LAGLIDADG intron-encoded homing endonuclease I-CreI, bound to homing site DNA has been determined. The interface is formed by, an extended, concave beta sheet from each enzyme monomer that contacts, each DNA half-site, resulting in direct side-chain contacts to 18 of the, 24 base pairs across the full-length homing site. The structure indicates, that I-CreI is optimized to its role in genetic transposition by, exhibiting long site-recognition while being able to cleave many closely, related target sequences. DNA cleavage is mediated by a compact pair of, active sites in the I-CreI homodimer, each of which contains a separate, bound divalent cation.

About this Structure

1BP7 is a Single protein structure of sequence from Chlamydomonas reinhardtii with CA as ligand. Full crystallographic information is available from OCA.

Reference

DNA recognition and cleavage by the LAGLIDADG homing endonuclease I-CreI., Jurica MS, Monnat RJ Jr, Stoddard BL, Mol Cell. 1998 Oct;2(4):469-76. PMID:9809068

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