1bq3
From Proteopedia
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SACCHAROMYCES CEREVISIAE PHOSPHOGLYCERATE MUTASE IN COMPLEX WITH INOSITOL HEXAKISPHOSPHATE
Overview
The effects that the inhibitors inositol hexakisphosphate and benzene, tri-, tetra- and hexacarboxylates have on the phosphoglycerate mutases, from Saccharomyces cerevisiae and Schizosaccharomyces pombe have been, determined. Their Kivalues have been calculated, and the ability of the, inhibitors to protect the enzymes against limited proteolysis, investigated. These biochemical data have been placed in a structural, context by the solution of the crystal structures of S. cerevisiae, phosphoglycerate mutase soaked with inositol hexakisphosphate or benzene, hexacarboxylate. These large polyanionic compounds bind to the enzyme so, as to block the entrance to the active-site cleft. They form multiple, interactions with the enzyme, consistent with their low Kivalues, and, afford good protection against limited proteolysis of the C-terminal, region by thermolysin. The inositol compound is more efficacious because, of its greater number of negative charges. The S. pombe phosphoglycerate, mutase that is inherently lacking a comparable C-terminal region has, higher Kivalues for the compounds tested. Moreover, the S. pombe enzyme is, less sensititive to proteolysis, and the presence or absence of the, inhibitor molecules has little effect on susceptibility to proteolysis.
About this Structure
1BQ3 is a Single protein structure of sequence from Saccharomyces cerevisiae with SO4 and IHP as ligands. Active as Phosphoglycerate mutase, with EC number 5.4.2.1 Full crystallographic information is available from OCA.
Reference
Polyanionic inhibitors of phosphoglycerate mutase: combined structural and biochemical analysis., Rigden DJ, Walter RA, Phillips SE, Fothergill-Gilmore LA, J Mol Biol. 1999 Jun 18;289(4):691-9. PMID:10369755
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