1bsr
From Proteopedia
|
BOVINE SEMINAL RIBONUCLEASE STRUCTURE AT 1.9 ANGSTROMS RESOLUTION
Overview
The crystal structure of bovine seminal ribonuclease, a homodimeric enzyme, closely related to pancreatic ribonuclease, has been refined at a nominal, resolution of 1.9 A employing data collected on an electronic area, detector. The final model consists of two chains containing 1990 non-H, atoms, seven sulfate anions and 113 water molecules per asymmetric unit., The unit-cell parameters are a = 36.5 (1), b = 66.7 (1) and c = 107.5 (2), A, space group P22(1)2(1). The R factor is 0.177 for 16 492 reflections in, the resolution range 6.0-1.9 A and the deviations from ideal values of, bond lengths and bond angles are 0.020 A and 3.7 degrees, respectively., The molecule is formed by two pancreatic like chains, which have their, N-terminal segments interchanged so that each active site is formed by, residues from both subunits. The two chains are related by a, non-crystallographic twofold symmetry and are covalently linked by two, consecutive disulfide bridges, which form an unusual sixteen-membered ring, across the dimer interface. The deviations from the molecular symmetry, the hydration shell and the sulfate-binding sites are also discussed in, relation to the known structure of the pancreatic enzyme.
About this Structure
1BSR is a Single protein structure of sequence from Bos taurus with SO4 as ligand. Full crystallographic information is available from OCA.
Reference
Bovine seminal ribonuclease: structure at 1.9 A resolution., Mazzarella L, Capasso S, Demasi D, Di Lorenzo G, Mattia CA, Zagari A, Acta Crystallogr D Biol Crystallogr. 1993 Jul 1;49(Pt 4):389-402. PMID:15299514
Page seeded by OCA on Tue Nov 20 11:53:34 2007
