1bw7
From Proteopedia
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SOLUTION STRUCTURE OF A DNA DUPLEX CONTAINING A REPLICABLE DIFLUOROTOLUENE-ADENINE PAIR
Overview
A nonpolar aromatic nucleoside derivative based on 2,4-difluorotoluene, (F), a non-hydrogen bonding shape analog of thymidine, was recently shown, to be replicated against adenine with high efficiency and fidelity. This, led to the suggestion that geometric matching, potentially even in the, absence of hydrogen bonding between bases in a pair, may be sufficient to, direct nucleotide selection during replication. We have examined the, solution structure of the F-A pair in the context of a 12 base pair DNA, duplex. We find that, despite the destabilization caused by this analog, the F-A pair very closely resembles that of a T x A pair in the same, context. This lends support to the importance of shape matching in, replication.
About this Structure
1BW7 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Solution structure of a DNA duplex containing a replicable difluorotoluene-adenine pair., Guckian KM, Krugh TR, Kool ET, Nat Struct Biol. 1998 Nov;5(11):954-9. PMID:9808039
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