1c9b
From Proteopedia
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CRYSTAL STRUCTURE OF A HUMAN TBP CORE DOMAIN-HUMAN TFIIB CORE DOMAIN COMPLEX BOUND TO AN EXTENDED, MODIFIED ADENOVIRAL MAJOR LATE PROMOTER (ADMLP)
Contents |
Overview
Transcription initiation requires the assembly of a preinitiation complex, (PIC), which is nucleated through binding of the TATA-box binding protein, (TBP) to the promoter. Biochemical studies have shown, however, that TBP, recognizes the TATA-box in both orientations and, therefore, cannot, account for the directionality of PIC assembly. Transcription factor IIB, (TFIIB) is essential for transcription initiation from RNA polymerase II, promoters. Recent functional studies have identified a specific 7 bp TFIIB, recognition element (BRE) immediately upstream of the TATA-box. We present, here the 2.65 A resolution crystal structure of a human TFIIBc-TBPc, complex bound to an idealized and extended adenovirus major late promoter., This structure now reveals that human TFIIBc binds to the promoter, asymmetrically through base-specific contacts in the major groove upstream, and in the minor groove downstream of the TATA-box. Binding of TFIIBc is, therefore, synergistic with TBPc requiring the distortion of the TATA-box., Thus, the newly described TFIIBc-DNA interface is likely to be a key, determinant for the unidirectional assembly of a functional PIC.
Disease
Known diseases associated with this structure: Huntington disease-like-4 OMIM:[600075], Parkinson disease OMIM:[600075], Spinocerebellar ataxia 17 OMIM:[600075]
About this Structure
1C9B is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural basis of preinitiation complex assembly on human pol II promoters., Tsai FT, Sigler PB, EMBO J. 2000 Jan 4;19(1):25-36. PMID:10619841
Page seeded by OCA on Mon Nov 12 16:18:37 2007
