1cbk
From Proteopedia
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7,8-DIHYDRO-6-HYDROXYMETHYLPTERIN-PYROPHOSPHOKINASE FROM HAEMOPHILUS INFLUENZAE
Overview
The gene encoding the 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, of Haemophilus influenzae has been cloned and expressed in Escherichia, coli. A complex of the purified protein with a substrate analog has been, crystallized and its structure solved by multiple anomalous dispersion, using phase information obtained from a single crystal of, selenomethione-labeled protein. The enzyme folds into a four-stranded, antiparallel beta-sheet flanked on one side by two alpha-helices and on, the other by three consecutive alpha-helices, giving a novel, beta1alpha1beta2beta3alpha2beta4alpha3alpha4alpha5 polypeptide topology., The three-dimensional structure of a binary complex has been refined at, 2.1 A resolution. The location of the substrate analog and a sulfate ion, gives important insight into the molecular mechanism of the enzyme.
About this Structure
1CBK is a Single protein structure of sequence from Haemophilus influenzae with SO4 and ROI as ligands. Full crystallographic information is available from OCA.
Reference
The structure and function of the 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase from Haemophilus influenzae., Hennig M, Dale GE, D'arcy A, Danel F, Fischer S, Gray CP, Jolidon S, Muller F, Page MG, Pattison P, Oefner C, J Mol Biol. 1999 Mar 26;287(2):211-9. PMID:10080886
Page seeded by OCA on Tue Nov 20 12:19:21 2007
