1cdt

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spinqualitylabelsall models 1cdt, resolution 2.5Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CARDIOTOXIN V4/II FROM NAJA MOSSAMBICA MOSSAMBICA: THE REFINED CRYSTAL STRUCTURE

Overview

The crystal structure of cardiotoxin VII4 from Naja mossambica mossambica, was refined to 2.5 A resolution. Fifty ordered solvent sites were, localized and included in the refinement. The final R factor is 0.197, (lambda/(2sin theta) less than 5 A; F greater than 3 sigma). The, three-dimensional structure is characterized by two beta-sheets. Of, particular interest is the two-stranded beta-sheet in the N-terminal, region. This shows a large right-handed twist and, though strongly, connected to the core of the molecule, and in particular to the C-terminal, end, protrudes out of the bulk of the molecule. The segment of four amino, acid residues connecting the two strands of this sheet is particularly, exposed. It contains an invariant proline residue that has probably an, important structural role, and is completely hydrophobic. Two other, conserved hydrophobic zones were identified; the largest extends over the, second and third loops, on one side only of the molecule. All side-chains, of invariant hydrophobic character (except proline residues) belong to one, of these three zones. Also discussed are the dimeric assembly and the, rather loose packing in the crystal. The three-dimensional structure is, compared with that of short and long alpha-neurotoxins. Comparison with, two-dimensional nuclear magnetic resonance results on the 68% homologous, cardiotoxin CT X IIb shows an excellent overall agreement. A few, differences are probably genuine.

About this Structure

1CDT is a Single protein structure of sequence from Naja mossambica with PO4 as ligand. Full crystallographic information is available from OCA.

Reference

Cardiotoxin VII4 from Naja mossambica mossambica. The refined crystal structure., Rees B, Bilwes A, Samama JP, Moras D, J Mol Biol. 1990 Jul 5;214(1):281-97. PMID:2370666

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