1cdz

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spinqualitylabelsall models 1cdz, resolution 3.2Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

BRCT DOMAIN FROM DNA-REPAIR PROTEIN XRCC1

Overview

The BRCT domain (BRCA1 C-terminus), first identified in the breast cancer, suppressor protein BRCA1, is an evolutionarily conserved protein-protein, interaction region of approximately 95 amino acids found in a large number, of proteins involved in DNA repair, recombination and cell cycle control., Here we describe the first three-dimensional structure and fold of a BRCT, domain determined by X-ray crystallography at 3.2 A resolution. The, structure has been obtained from the C-terminal region of the human DNA, repair protein XRCC1, and comprises a four-stranded parallel beta-sheet, surrounded by three alpha-helices, which form an autonomously folded, domain. The compact XRCC1 structure explains the observed sequence, homology between different BRCT motifs and provides a framework for, modelling other BRCT domains. Furthermore, the established structure of an, XRCC1 BRCT homodimer suggests potential protein-protein interaction sites, for the complementary BRCT domain in DNA ligase III, since these two, domains form a stable heterodimeric complex. Based on the XRCC1 BRCT, structure, we have constructed a model for the C-terminal BRCT domain of, BRCA1, which frequently is mutated in familial breast and ovarian cancer., The model allows insights into the effects of such mutations on the fold, of the BRCT domain.

About this Structure

1CDZ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of an XRCC1 BRCT domain: a new protein-protein interaction module., Zhang X, Morera S, Bates PA, Whitehead PC, Coffer AI, Hainbucher K, Nash RA, Sternberg MJ, Lindahl T, Freemont PS, EMBO J. 1998 Nov 2;17(21):6404-11. PMID:9799248

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