1cgp
From Proteopedia
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CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE
Overview
The 3 angstrom resolution crystal structure of the Escherichia coli, catabolite gene activator protein (CAP) complexed with a 30-base pair DNA, sequence shows that the DNA is bent by 90 degrees. This bend results, almost entirely from two 40 degrees kinks that occur between TG/CA base, pairs at positions 5 and 6 on each side of the dyad axis of the complex., DNA sequence discrimination by CAP derives both from sequence-dependent, distortion of the DNA helix and from direct hydrogen-bonding interactions, between three protein side chains and the exposed edges of three base, pairs in the major groove of the DNA. The structure of this transcription, factor--DNA complex provides insights into possible mechanisms of, transcription activation.
About this Structure
1CGP is a Single protein structure of sequence from Escherichia coli with CMP as ligand. The following page contains interesting information on the relation of 1CGP with [Catabolite Activator Protein]. Full crystallographic information is available from OCA.
Reference
Crystal structure of a CAP-DNA complex: the DNA is bent by 90 degrees., Schultz SC, Shields GC, Steitz TA, Science. 1991 Aug 30;253(5023):1001-7. PMID:1653449
Page seeded by OCA on Sun Nov 18 08:58:22 2007
