1ctp
From Proteopedia
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STRUCTURE OF THE MAMMALIAN CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE AND AN INHIBITOR PEPTIDE DISPLAYS AN OPEN CONFORMATION
Overview
The crystal structure of a binary complex of the porcine heart catalytic, (C) subunit of cAMP-dependent protein kinase (space group P4(1)32; a =, 171.5 A) complexed with a di-iodinated peptide inhibitor, PKI(5-24), has, been solved and refined to 2.9 A resolution with an overall R of 21.1%., The r.m.s. deviations from ideal bond lengths and angles are 0.022 A and, 4.3 degrees. A single isotropic B of 17 A(2) was used for all atoms. The, structure solution was carried out initially by molecular replacement of, electron density followed by refinement against atomic coordinates from, orthorhombic crystals of a binary complex of the mouse recombinant enzyme, previously described [Knighton, Zheng, Ten Eyck, Ashford, Xuong, Taylor &, Sowadski (1991). Science, 253, 407-414]. The most striking difference, between the two crystal structures is a large displacement of the small, lobe of the enzyme. In the cubic crystal, the beta-sheet of the small lobe, is rotated by 15 degrees and translated by 1.9 A with respect to the, orthorhombic crystal. Possible explanations for why this binary complex, crystallized in an open conformation in contrast to a similar binary, complex of the recombinant enzyme are discussed. This study demonstrates, that considerable information about parts of a crystal structure can be, obtained without a complete crystal structure analysis. Specifically, the, six rigid-group parameters of a poly alanine model of the beta-structure, were obtained satisfactorily from a crystal structure by refinement of, difference Fourier coefficients based on an approximate partial structure, model.
About this Structure
1CTP is a Protein complex structure of sequences from Sus scrofa with PO3, MYR and TYI as ligands. Active as Non-specific serine/threonine protein kinase, with EC number 2.7.11.1 Full crystallographic information is available from OCA.
Reference
Structure of the mammalian catalytic subunit of cAMP-dependent protein kinase and an inhibitor peptide displays an open conformation., Karlsson R, Zheng J, Xuong N, Taylor SS, Sowadski JM, Acta Crystallogr D Biol Crystallogr. 1993 Jul 1;49(Pt 4):381-8. PMID:15299513
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