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1a7i

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1a7i

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AMINO-TERMINAL LIM DOMAIN FROM QUAIL CYSTEINE AND GLYCINE-RICH PROTEIN, NMR, MINIMIZED AVERAGE STRUCTURE

Overview

Members of the cysteine and glycine-rich protein (CRP) family (CRP1, CRP2, and CRP3) contain two zinc-binding LIM domains, LIM1 and LIM2, and are, implicated in diverse cellular processes linked to differentiation, growth, control and pathogenesis. The solution structure of an 81-amino acid, recombinant peptide encompassing the amino-terminal LIM1 domain of quail, CRP2 has been determined by 2D and 3D homo- and heteronuclear NMR, spectroscopy. The LIM1 domain consists of two zinc binding sites of the, CCHC and the CCCC type, respectively, which both contain two orthogonally, arranged antiparallel beta-sheets and which are packed together by a, hydrophobic core composed of residues from the zinc finger loop regions., The CCCC zinc finger is followed by a short alpha-helical stretch. The, ... [(full description)]

About this Structure

1A7I is a [Single protein] structure of sequence from [Coturnix coturnix japonica] with ZN as [ligand]. Full crystallographic information is available from [OCA].

Reference

Structure and intramodular dynamics of the amino-terminal LIM domain from quail cysteine- and glycine-rich protein CRP2., Kontaxis G, Konrat R, Krautler B, Weiskirchen R, Bister K, Biochemistry. 1998 May 19;37(20):7127-34. PMID:9585524

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