1d2f

Categories: Escherichia coli | Single protein | Clausen, T. | PLP | Aminotransferase fold | Large plp-binding domain | Open alpha-beta structure. | Small c-terminal domain

X-RAY STRUCTURE OF MALY FROM ESCHERICHIA COLI: A PYRIDOXAL-5'-PHOSPHATE-DEPENDENT ENZYME ACTING AS A MODULATOR IN MAL GENE EXPRESSION

Overview

MalY represents a bifunctional pyridoxal 5'-phosphate-dependent enzyme, acting as a beta-cystathionase and as a repressor of the maltose regulon., Here we present the crystal structures of wild-type and A221V mutant, protein. Each subunit of the MalY dimer is composed of a large pyridoxal, 5'-phosphate-binding domain and a small domain similar to, aminotransferases. The structural alignment with related enzymes, identifies residues that are generally responsible for beta-lyase activity, and depicts a unique binding mode of the pyridoxal 5'-phosphate correlated, with a larger, more flexible substrate-binding pocket. In a screen for, MalY mutants with reduced mal repressor properties, mutations occurred in, three clusters: I, 83-84; II, 181-189 and III, 215-221, which constitute a, clearly distinguished region in the MalY crystal structure far away from, the cofactor. The tertiary structure of one of these mutants (A221V), demonstrates that positional rearrangements are indeed restricted to, regions I, II and III. Therefore, we propose that a direct protein-protein, interaction with MalT, the central transcriptional activator of the, maltose system, underlies MalY-dependent repression of the maltose system.

About this Structure

1D2F is a Single protein structure of sequence from Escherichia coli with PLP as ligand. Full crystallographic information is available from OCA.

Reference

X-ray structure of MalY from Escherichia coli: a pyridoxal 5'-phosphate-dependent enzyme acting as a modulator in mal gene expression., Clausen T, Schlegel A, Peist R, Schneider E, Steegborn C, Chang YS, Haase A, Bourenkov GP, Bartunik HD, Boos W, EMBO J. 2000 Mar 1;19(5):831-42. PMID:10698925

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