1d32
From Proteopedia
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DRUG-INDUCED DNA REPAIR: X-RAY STRUCTURE OF A DNA-DITERCALINIUM COMPLEX
Overview
Ditercalinium is a synthetic anticancer drug that binds to DNA by, bis-intercalation and activates DNA repair processes. In prokaryotes, noncovalent DNA-ditercalinium complexes are incorrectly recognized by the, uvrABC repair system as covalent lesions on DNA. In eukaryotes, mitochondrial DNA is degraded by excess and futile DNA repair. Using x-ray, crystallography, we have determined, to 1.7 A resolution, the, three-dimensional structure of a complex of ditercalinium bound to the, double-stranded DNA fragment [d(CGCG)]2. The DNA in the complex with, ditercalinium is kinked (by 15 degrees) and severely unwound (by 36, degrees) with exceptionally wide major and minor grooves. Recognition of, the DNA-ditercalinium complex by uvrABC in prokaryotes, and by, mitochondrial DNA repair systems in eukaryotes, might be related to, drug-induced distortion of the DNA helix.
About this Structure
1D32 is a Protein complex structure of sequences from [1] with DIT as ligand. Full crystallographic information is available from OCA.
Reference
Drug-induced DNA repair: X-ray structure of a DNA-ditercalinium complex., Gao Q, Williams LD, Egli M, Rabinovich D, Chen SL, Quigley GJ, Rich A, Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2422-6. PMID:2006181
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