This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1d9m
From Proteopedia
|
SOLUTION STRUCTURE OF CECROPIN A(1-8)-MAGAININ 2 HYBRID PEPTIDE ANALOGUE(P2)
Overview
In order to elucidate the structure-antibiotic activity relationships of, the peptides, the three-dimensional structures of two hybrid peptides, CA(1-8) - MA(1-12) and CA(1-8) - ME(1-12) in trifluoroethanol-containing, aqueous solution were investigated by NMR spectroscopy. Both CA(1-8) -, MA(1-12) and CA(1-8) - ME(1-12) have strong antibacterial activity but, only CA(1-8) - ME(1-12) has hemolytic activity against human erythrocytes., CA(1-8) - MA(1-12) has a hydrophobic 310-helix of only two turns combined, with one short helix in the N-terminus with a flexible hinge section in, between. CA(1-8) - MA(1-12) has a severely bent structure in the middle of, the peptide. These structural features as well as the low hydrophobicity, of CA(1-8) - MA(1-12) seem to be crucial for the selective lysis against, the membrane of prokaryotic cells. CA(1-8) - ME(1-12) has an alpha-helical, structure of about three turns in the melittin domain and a flexible, structure with one turn in the cecropin domain connected with a flexible, hinge section in between, and these might be the structural features, required for membrane disruption against prokaryotic and eukaryotic cells., The central hinge region (Gly9-Ile10-Gly11) in an amphipathic, antibacterial peptide is considered to play an important role in providing, the conformational flexibility required for ion channel formation of the, C-terminal hydrophobic alpha-helix on cell membrane.
About this Structure
1D9M is a Single protein structure of sequence from Xenopus laevis + hyalophora cecropia with NH2 as ligand. Full crystallographic information is available from OCA.
Reference
NMR structural characterization of cecropin A(1-8) - magainin 2(1-12) and cecropin A (1-8) - melittin (1-12) hybrid peptides., Oh D, Shin SY, Kang JH, Hahm KS, Kim KL, Kim Y, J Pept Res. 1999 May;53(5):578-89. PMID:10424354
Page seeded by OCA on Tue Nov 20 13:06:19 2007
