1dpt

From Proteopedia

proteopedia linkproteopedia link

spinqualitylabelsall models 1dpt, resolution 1.54Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

D-DOPACHROME TAUTOMERASE

Overview

D-Dopachrome tautomerase shares a low homologous amino acid sequence (33%, homology) with the macrophage migration inhibitory factor (MIF) and, possesses similar tautomerase activity as well. MIF is a cytokine involved, in inflammatory reactions and immune responses. Whereas recent studies, have identified MIF as a pituitary hormone and immunoregulator, much less, is known about the structural basis of these physiological functions and, the real significance of tautomerase activity. Therefore, interest in the, structure-function relationship between D-dopachrome tautomerase and MIF, has increased, especially with regard to inflammation and immune, responses. We have determined the X-ray crystal structure of human, D-dopachrome tautomerase at 1.54 A resolution. D-Dopachrome tautomerase, folds to form a homotrimer that has extensive contact between subunits by, intersubunit beta-sheets. Its overall topology and trimeric formations are, similar to those of human MIF. The N-terminal proline is located at the, bottom of a positively charged pocket in which the conformations of Lys32, and Ser63 are highly conserved. These positively charged properties are, also seen in the active site pocket of human MIF, bacterial, 5-(carboxymethyl)-2-hydroxymuconate isomerase (CHMI), and 4-oxalocrotonate, tautomerase (4-OT). A detailed comparison of these structures revealed, significant differences in the environment around the potential active, site, the intersubunit contacts, and charge distribution on the molecular, surface. It can be concluded that these features are related to the, physiological role and tautomerase activity of MIF and D-dopachrome, tautomerase. The present structural study could be helpful for designing, effective inhibitors that modulate immunoregulatory and hormone-like, effects.

About this Structure

1DPT is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of human D-dopachrome tautomerase, a homologue of macrophage migration inhibitory factor, at 1.54 A resolution., Sugimoto H, Taniguchi M, Nakagawa A, Tanaka I, Suzuki M, Nishihira J, Biochemistry. 1999 Mar 16;38(11):3268-79. PMID:10079069

Page seeded by OCA on Mon Nov 12 16:34:49 2007