1dsn

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spinqualitylabelsall models 1dsn, resolution 2.05Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

D60S N-TERMINAL LOBE HUMAN LACTOFERRIN

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

The crystal structure of a site-specific mutant of the N-terminal, half-molecule of human lactoferrin, Lf(N), in which the iron ligand Asp60, has been mutated to Ser, has been determined at 2.05 A resolution in order, to determine the effects of the mutation on iron binding and domain, closure. Yellow monoclinic crystals of the D60S mutant, in its iron-bound, form, were prepared, and have unit cell dimensions a = 110.2 A, b = 57.0, A, c = 55.2 A, beta = 97.6 degrees, space group C2, with one molecule of, 333 residues in the asymmetric unit. The structure was determined by, molecular replacement, using the wild-type Lf(N) as search model, and was, refined by restrained least-squares methods. The final model, comprising, 2451 protein atoms (from residues 2 to 315) one Fe3+ and one CO2-(3), and, 107 water molecules, gives an R-factor of 0.175 for all data in the, resolution range 20.0 to 2.05 A. The model conforms well with standard, geometry, having root-mean-square deviations of 0.014 A and 1.2 degrees, from standard bond lengths and angles. The structure of the D60S mutant, deviates in two important respects from the parent Lf(N) molecule. At the, mutation site the Ser side-chain neither binds to the iron atom nor makes, any interdomain contact as the substituted Asp does; instead a water, molecule fills the iron coordination site and participates in interdomain, hydrogen bonding. The domain closure is also changed, with the D60S mutant, having a more closed conformation. Consideration of crystal packing, suggests that the altered domain closure is a genuine molecular property, but both the iron coordination and interdomain contacts are consistent, with weakened iron binding in the mutant. The implications for iron, binding in transferrins generally are discussed.

Disease

Known disease associated with this structure: Deafness, autosomal dominant 1 OMIM:[602121]

About this Structure

1DSN is a Single protein structure of sequence from Homo sapiens with FE and CO3 as ligands. Full crystallographic information is available from OCA.

Reference

Altered domain closure and iron binding in transferrins: the crystal structure of the Asp60Ser mutant of the amino-terminal half-molecule of human lactoferrin., Faber HR, Bland T, Day CL, Norris GE, Tweedie JW, Baker EN, J Mol Biol. 1996 Feb 23;256(2):352-63. PMID:8594202

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