1e4p
From Proteopedia
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STRUCTURE OF THE RIBOZYME SUBSTRATE HAIRPIN OF NEUROSPORA VS RNA. A CLOSE LOOK AT THE CLEAVAGE SITE
Overview
The cleavage site of the Neurospora VS RNA ribozyme is located in a, separate hairpin domain containing a hexanucleotide internal loop with an, A-C mismatch and two adjacent G-A mismatches. The solution structure of, the internal loop and helix la of the ribozyme substrate hairpin has been, determined by nuclear magnetic resonance (NMR) spectroscopy. The 2 nt in, the internal loop, flanking the cleavage site, a guanine and adenine, are, involved in two sheared G.A base pairs similar to the magnesium, ion-binding site of the hammerhead ribozyme. Adjacent to the tandem G.A, base pairs, the adenine and cytidine, which are important for cleavage, form a noncanonical wobble A+-C base pair. The dynamic properties of the, internal loop and details of the high-resolution structure support the, view that the hairpin structure represents a ground state, which has to, undergo a conformational change prior to cleavage. Results of chemical, modification and mutagenesis data of the Neurospora VS RNA ribozyme can be, explained in context with the present three-dimensional structure.
About this Structure
1E4P is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structure of the ribozyme substrate hairpin of Neurospora VS RNA: a close look at the cleavage site., Michiels PJ, Schouten CH, Hilbers CW, Heus HA, RNA. 2000 Dec;6(12):1821-32. PMID:11142381
Page seeded by OCA on Sat Nov 24 21:45:30 2007
