1w9h
From Proteopedia
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THE STRUCTURE OF A PIWI PROTEIN FROM ARCHAEOGLOBUS FULGIDUS.
Overview
RNA silencing regulates gene expression through mRNA degradation, translation repression and chromatin remodelling. The fundamental engines, of RNA silencing are RISC and RITS complexes, whose common components are, 21-25 nt RNA and an Argonaute protein containing a PIWI domain of unknown, function. The crystal structure of an archaeal Piwi protein (AfPiwi) is, organised into two domains, one resembling the sugar-binding portion of, the lac repressor and another with similarity to RNase H. Invariant, residues and a coordinated metal ion lie in a pocket that surrounds the, conserved C-terminus of the protein, defining a key functional region in, the PIWI domain. Furthermore, two Asp residues, conserved in the majority, of Argonaute sequences, align spatially with the catalytic Asp residues ... [(full description)]
About this Structure
1W9H is a [Single protein] structure of sequence from [Archaeoglobus fulgidus] with CD, NI and CL as [ligands]. Full crystallographic information is available from [OCA].
Reference
Crystal structure of a PIWI protein suggests mechanisms for siRNA recognition and slicer activity., Parker JS, Roe SM, Barford D, EMBO J. 2004 Dec 8;23(24):4727-37. Epub 2004 Nov 25. PMID:15565169
Page seeded by OCA on Mon Oct 29 21:22:36 2007
Categories: Archaeoglobus fulgidus | Single protein | Barford, D. | Parker, J.S. | Roe, S.M. | CD | CL | NI | Argonaute | Nuclear protein | Piwi domain | Risc | Rnai
