1fca
From Proteopedia
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STRUCTURE OF THE FERREDOXIN FROM CLOSTRIDIUM ACIDURICI: MODEL AT 1.8 ANGSTROMS RESOLUTION
Overview
Ferredoxins (Fd) are electron-carrier proteins, the active sites of which, are organized around clusters made of iron and inorganic sulfur. The Fd, from Clostridium acidurici is 55 amino acids long and contains two, [4Fe-4S] clusters. Crystals have been obtained in the space group, P4(3)2(1)2, a = b = 34.441 (5), c = 74.778 (9) A. The structure was solved, by molecular replacement using the Fd from Peptostreptcoccus, asaccharolyticus as a search model, these two ferredoxins having 37, residues in common. Refinement using molecular-dynamics techniques was, then initiated. Successive rounds of model building and refinement gave a, structure that includes 45 water molecules with R = 15%. At this stage, the electron-density map clearly revealed discrepancies in the position of, two amino acids in the published primary sequence. Refinement based on, these modifications led to R = 14.3% for 3921 reflections up to 1.8 A, resolution. The geometry of the two clusters has been found to be in good, agreement with that previously obtained at a lower resolution., Interactions of polypeptide chain with the [4Fe-4S] clusters, the cluster, geometry as well as the hydrogen bonds involving S, Sgamma, N and water, molecules are reported.
About this Structure
1FCA is a Single protein structure of sequence from Clostridium acidurici with SF4 as ligand. Full crystallographic information is available from OCA.
Reference
Structure of the ferredoxin from Clostridium acidurici: model at 1.8 A resolution., TranQui D, Jesior JC, Acta Crystallogr D Biol Crystallogr. 1995 Mar 1;51(Pt 2):155-9. PMID:15299316
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