1fr3
From Proteopedia
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THE HIGH RESOLUTION STRUCTURE OF A MOLYBDATE BINDING PROTEIN FROM SPOROMUSA OVATA
Overview
BACKGROUND: Transport of molybdenum into bacteria involves a high-affinity, ABC transporter system whose expression is controlled by a repressor, protein called ModE. While molybdate transport is tightly coupled to, utilization in some bacteria, other organisms have molybdenum storage, proteins. One class of putative molybdate storage proteins is, characterized by a sequence consisting of about 70 amino acids (Mop). A, tandem repeat of Mop sequences also constitutes the molybdate binding, domain of ModE. RESULTS: We have determined the crystal structure of the 7, kDa Mop protein from the methanol-utilizing anaerobic eubacterium, Sporomusa ovata grown in the presence of molybdate and tungstate. The, protein occurs as highly symmetric hexamers binding eight oxyanions. Each, peptide assumes a so-called OB fold, which has previously also been, observed in ModE. There are two types of oxyanion binding sites in Mo at, the interface between two or three peptides. All oxyanion binding sites, were found to be occupied by WO(4) rather than MoO(4). CONCLUSIONS: The, biological function of proteins containing only Mop sequences is unknown, but they have been implicated in molybdate homeostasis and molybdopterin, cofactor biosynthesis. While there are few indications that the S. ovata, Mop binds pterin, the structure suggests that only the type-1 oxyanion, binding sites would be sufficiently accessible to bind a cofactor. The, observed occupation of the oxyanion binding sites by WO(4) indicates that, Mop might also be involved in controlling intracellular tungstate levels.
About this Structure
1FR3 is a Single protein structure of sequence from Sporomusa ovata with WO4 as ligand. Full crystallographic information is available from OCA.
Reference
Structure of the molybdate/tungstate binding protein mop from Sporomusa ovata., Wagner UG, Stupperich E, Kratky C, Structure. 2000 Nov 15;8(11):1127-36. PMID:11080635
Page seeded by OCA on Sun Nov 25 00:15:08 2007
