1fuj

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spinqualitylabelsall models 1fuj, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

PR3 (MYELOBLASTIN)

Overview

The crystal structure of PR3, a serine proteinase from the azurophilic, granules of human polymorphonuclear neutrophils, has been solved by, molecular replacement using the human leukocyte elastase structure. The, PR3 structure has been refined to an R-factor (= sigma parallel Fo, magnitude of-Fc parallel/sigma magnitude of Fo) of 0.201 for all data in, the range of 10.0 to 2.2 A resolution. The enzyme was crystallized in, space group P21 with four molecules in the asymmetric unit (Vm, approximately equal to 2.6 A/Da). The overall fold consists of two domains, of beta-barrel structures typical of the chymotrypsin family of serine, proteinases. In general, the substrate binding sites, S4 to S3', are more, polar than comparable sites in the related proteinase, human leukocyte, elastase. The experimentally observed preference of PR3 for small, aliphatic residues at the P1 position of a substrate is explained by the, Val to Ile substitution at position 190 when compared to the elastase, structure. The substitution of Ala by Asp at position 213 at the back of, S1 should not affect its specificity greatly, as the Asp side-chain points, back into the interior of the protein. The PR3 structure includes a, disaccharide unit (N-linked 2-acetamido-2-deoxy-beta-D-glucopyranose and, 1,6-linked alpha-L-fucopyranose) covalently attached to Asn 159. The, linear antigenic sites of PR3 reported to react with Wegener's, granulomatosis autoantibodies occur in regions of the three-dimensional, structure that may implicate the inactive pro-form of the enzyme in the, pathogenesis of the disease.

About this Structure

1FUJ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The crystal structure of PR3, a neutrophil serine proteinase antigen of Wegener's granulomatosis antibodies., Fujinaga M, Chernaia MM, Halenbeck R, Koths K, James MN, J Mol Biol. 1996 Aug 16;261(2):267-78. PMID:8757293

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