1fxa
From Proteopedia
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CRYSTALLIZATION AND STRUCTURE DETERMINATION TO 2.5-ANGSTROMS RESOLUTION OF THE OXIDIZED [2FE-2S] FERREDOXIN ISOLATED FROM ANABAENA 7120
Overview
The molecular structure of the oxidized form of the [2Fe-2S] ferredoxin, isolated from the cyanobacterium Anabaena species strain PCC 7120 has been, determined by X-ray diffraction analysis to a nominal resolution of 2.5 A, and refined to a crystallographic R factor of 18.7%. Crystals used in this, investigation belong to the space group P2(1)2(1)2(1) with unit cell, dimensions of a = 37.42 A, b = 38.12 A, and c = 147.12 A and two molecules, in the asymmetric unit. The three-dimensional structure of this ferredoxin, was solved by a method that combined X-ray data from one isomorphous, heavy-atom derivative with noncrystallographic symmetry averaging and, solvent flattening. As in other plant-type [2Fe-2S] ferredoxins, the, iron-sulfur cluster is located toward the outer edge of the molecule, and, the irons are tetrahedrally coordinated by both inorganic sulfurs and, sulfurs provided by protein cysteine residues. The main secondary, structural elements include four strands of beta-pleated sheet and three, alpha-helical regions.
About this Structure
1FXA is a Single protein structure of sequence from Anabaena sp. with FES as ligand. Full crystallographic information is available from OCA.
Reference
Crystallization and structure determination to 2.5-A resolution of the oxidized [2Fe-2S] ferredoxin isolated from Anabaena 7120., Rypniewski WR, Breiter DR, Benning MM, Wesenberg G, Oh BH, Markley JL, Rayment I, Holden HM, Biochemistry. 1991 Apr 30;30(17):4126-31. PMID:1902376
Page seeded by OCA on Tue Nov 20 15:25:52 2007
