1fxz
From Proteopedia
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CRYSTAL STRUCTURE OF SOYBEAN PROGLYCININ A1AB1B HOMOTRIMER
Overview
Soybean glycinin is a member of the 11 S globulin family. The crystal, structure of proglycinin was determined by X-ray crystallography at 2.8 A, resolution with an R-factor of 0.199 and a free R-factor of 0.250. A, trimer molecule was found in an asymmetric unit of crystals. The trimer, model contains three A1aB1b subunits and comprises 1128 amino acid, residues and 34 water molecules. The constituent protomers of the, homo-trimeric protein are arranged around a 3-fold symmetry axis with, dimensions of 95 Ax95 Ax40 A. The protomer model is composed of five, fragments which correspond roughly to conserved regions based on the, sequence alignment of various 11 S globulins. The core of the protomer, consists of two jelly-roll beta-barrels and two extended helix domains., This structure of proglycinin is similar to those of canavalin and, phaseolin belonging to the 7 S globulin family, strongly supporting the, hypothesis that both 7 S and 11 S globulins are derived from a common, ancestor. The inter and intra-chain disulfide bonds conserved in the 11 S, globulin family are clearly observed. It is found that the face with the, inter-chain disulfide bond (IE face) contains more hydrophobic residues, than that with the intra-chain disulfide bond. This suggests that a mature, hexamer is formed by the interaction between the IE faces after, processing.
About this Structure
1FXZ is a Single protein structure of sequence from Glycine max. Full crystallographic information is available from OCA.
Reference
Crystal structure of soybean proglycinin A1aB1b homotrimer., Adachi M, Takenaka Y, Gidamis AB, Mikami B, Utsumi S, J Mol Biol. 2001 Jan 12;305(2):291-305. PMID:11124907
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