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1fze
From Proteopedia
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CRYSTAL STRUCTURE OF FRAGMENT DOUBLE-D FROM HUMAN FIBRIN
Contents |
Overview
The structure of fragment double-D from human fibrin has been solved in, the presence and absence of the peptide ligands that simulate the two, knobs exposed by the removal of fibrinopeptides A and B, respectively. All, told, six crystal structures have been determined, three of which are, reported here for the first time: namely, fragments D and double-D with, the peptide GHRPam alone and double-D in the absence of any peptide, ligand. Comparison of the structures has revealed a series of, conformational changes that are brought about by the various knob-hole, interactions. Of greatest interest is a moveable "flap" of two negatively, charged amino acids (Glubeta397 and Aspbeta398) whose side chains are, pinned back to the coiled coil with a calcium atom bridge until GHRPam, occupies the beta-chain pocket. Additionally, in the absence of the, peptide ligand GPRPam, GHRPam binds to the gamma-chain pocket, a new, calcium-binding site being formed concomitantly.
Disease
Known diseases associated with this structure: Afibrinogenemia, congenital OMIM:[134820], Afibrinogenemia, congenital OMIM:[134830], Amyloidosis, hereditary renal OMIM:[134820], Dysfibrinogenemia, alpha type, causing bleeding diathesis OMIM:[134820], Dysfibrinogenemia, alpha type, causing recurrent thrombosis OMIM:[134820], Dysfibrinogenemia, beta type OMIM:[134830], Dysfibrinogenemia, gamma type OMIM:[134850], Hypofibrinogenemia, gamma type OMIM:[134850], Thrombophilia, dysfibrinogenemic OMIM:[134830], Thrombophilia, dysfibrinogenemic OMIM:[134850]
About this Structure
1FZE is a Protein complex structure of sequences from Homo sapiens with NAG and CA as ligands. Full crystallographic information is available from OCA.
Reference
Conformational changes in fragments D and double-D from human fibrin(ogen) upon binding the peptide ligand Gly-His-Arg-Pro-amide., Everse SJ, Spraggon G, Veerapandian L, Doolittle RF, Biochemistry. 1999 Mar 9;38(10):2941-6. PMID:10074346
Page seeded by OCA on Mon Nov 12 16:58:48 2007
Categories: Homo sapiens | Protein complex | Doolittle, R.F. | Everse, S.J. | Spraggon, G. | Veerapandian, L. | CA | NAG | Blood coagulation | Fibrin | Fibrinogen | Plasma | Platelet
