1g9r
From Proteopedia
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CRYSTAL STRUCTURE OF GALACTOSYLTRANSFERASE LGTC IN COMPLEX WITH MN AND UDP-2F-GALACTOSE
Overview
Many bacterial pathogens express lipooligosaccharides that mimic human, cell surface glycoconjugates, enabling them to attach to host receptors, and to evade the immune response. In Neisseria meningitidis, the, galactosyltransferase LgtC catalyzes a key step in the biosynthesis of, lipooligosaccharide structure by transferring alpha-d-galactose from, UDP-galactose to a terminal lactose. The product retains the configuration, of the donor sugar glycosidic bond; LgtC is thus a retaining, glycosyltranferase. We report the 2 A crystal structures of the complex of, LgtC with manganese and UDP 2-deoxy-2-fluoro-galactose (a donor sugar, analog) in the presence and absence of the acceptor sugar analog, 4'-deoxylactose. The structures, together with results from site-directed, mutagenesis and kinetic analysis, give valuable insights into the unique, catalytic mechanism and, as the first structure of a glycosyltransferase, in complex with both the donor and acceptor sugars, provide a starting, point for inhibitor design.
About this Structure
1G9R is a Single protein structure of sequence from Neisseria meningitidis with MN, UPF and ACY as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of the retaining galactosyltransferase LgtC from Neisseria meningitidis in complex with donor and acceptor sugar analogs., Persson K, Ly HD, Dieckelmann M, Wakarchuk WW, Withers SG, Strynadka NC, Nat Struct Biol. 2001 Feb;8(2):166-75. PMID:11175908
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