1ga1
From Proteopedia
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CRYSTAL STRUCTURE ANALYSIS OF PSCP (PSEUDOMONAS SERINE-CARBOXYL PROTEINASE) COMPLEXED WITH A FRAGMENT OF IODOTYROSTATIN (THIS ENZYME RENAMED "SEDOLISIN" IN 2003)
Overview
The crystal structure of pepstatin-insensitive carboxyl proteinase (PCP), from Pseudomonas sp. 101, an enzyme with no overall sequence similarity to, any other proteinases of known structure, was solved using crystals soaked, in sodium bromide solution and then cryocooled. A data set collected at, the bromine peak absorption wavelength was sufficient for calculation of, an excellent map and the entire process of phasing and tracing the maps, required almost no direct human intervention. The process of structure, solution using single-wavelength data was compared with three-wavelength, multiwavelength anomalous diffraction (MAD); although the latter resulted, in slightly better maps, the use of this much more labor-intensive, approach did not significantly improve the ability to solve the structure., The successful phasing approaches are compared with several less, successful attempts utilizing other crystal forms of the enzyme and the, practical aspects of the use of bromine as a heavy-atom derivative are, discussed. In conclusion, the use of halides with single-wavelength, diffraction data fulfills the requirements of being a first-choice method, of high-throughput structure solution for the emerging field of structural, genomics.
About this Structure
1GA1 is a Single protein structure of sequence from Pseudomonas sp. with CA, CL and GOL as ligands. Active as Pseudomonalisin, with EC number 3.4.21.100 Full crystallographic information is available from OCA.
Reference
Practical experience with the use of halides for phasing macromolecular structures: a powerful tool for structural genomics., Dauter Z, Li M, Wlodawer A, Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):239-49. PMID:11173470
Page seeded by OCA on Tue Nov 20 15:52:12 2007
Categories: Pseudomonalisin | Pseudomonas sp. | Single protein | Dauter, Z. | Li, M. | Wlodawer, A. | CA | CL | GOL | Serine-carboxyl proteinase
