1gpe

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spinqualitylabelsall models 1gpe, resolution 1.8Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

GLUCOSE OXIDASE FROM PENICILLIUM AMAGASAKIENSE

Overview

Glucose oxidase is a flavin-dependent enzyme which catalyses the oxidation, of beta-D-glucose by molecular oxygen to delta-gluconolactone and hydrogen, peroxide. The structure of the enzyme from Aspergillus niger, previously, refined at 2.3 A resolution, has been refined at 1.9 A resolution to an R, value of 19.0%, and the structure of the enzyme from Penicillium, amagasakiense, which has 65% sequence identity, has been determined by, molecular replacement and refined at 1.8 A resolution to an R value of, 16.4%. The structures of the partially deglycosylated enzymes have an, r.m.s. deviation of 0.7 A for main-chain atoms and show four, N-glycosylation sites, with an extended carbohydrate moiety at Asn89., Substrate complexes of the enzyme from A. niger were modelled by, force-field methods. The resulting model is consistent with results from, site-directed mutagenesis experiments and shows the beta-D-glucose, molecule in the active site of glucose oxidase, stabilized by 12 hydrogen, bonds and by hydrophobic contacts to three neighbouring aromatic residues, and to flavin adenine dinucleotide. Other hexoses, such as, alpha-D-glucose, mannose and galactose, which are poor substrates for the, enzyme, and 2-deoxy-D-glucose, form either fewer bonds or unfavourable, contacts with neighbouring amino acids. Simulation of the complex between, the reduced enzyme and the product, delta-gluconolactone, has provided an, explanation for the lack of product inhibition by the lactone.

About this Structure

1GPE is a Single protein structure of sequence from Penicillium amagasakiense with NAG and FAD as ligands. The following page contains interesting information on the relation of 1GPE with [Glucose Oxidase]. Active as Glucose oxidase, with EC number 1.1.3.4 Full crystallographic information is available from OCA.

Reference

1.8 and 1.9 A resolution structures of the Penicillium amagasakiense and Aspergillus niger glucose oxidases as a basis for modelling substrate complexes., Wohlfahrt G, Witt S, Hendle J, Schomburg D, Kalisz HM, Hecht HJ, Acta Crystallogr D Biol Crystallogr. 1999 May;55(Pt 5):969-77. PMID:10216293

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