1gqi
From Proteopedia
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STRUCTURE OF PSEUDOMONAS CELLULOSA ALPHA-D-GLUCURONIDASE
Overview
Alpha-glucuronidases, components of an ensemble of enzymes central to the, recycling of photosynthetic biomass, remove the alpha-1,2 linked, 4-O-methyl glucuronic acid from xylans. The structure of the, alpha-glucuronidase, GlcA67A, from Pseudomonas cellulosa reveals three, domains, the central of which is a (beta/alpha)(8) barrel housing the, catalytic apparatus. Complexes of the enzyme with the individual reaction, products, either xylobiose or glucuronic acid, and the ternary complex of, both glucuronic acid and xylotriose reveal a "blind" pocket which selects, for short decorated xylooligosaccharides substituted with the uronic acid, at their nonreducing end, consistent with kinetic data. The catalytic, center reveals a constellation of carboxylates; Glu292 is poised to, provide protonic assistance to leaving group departure with Glu393 and, Asp365 both appropriately positioned to provide base-catalyzed assistance, for inverting nucleophilic attack by water.
About this Structure
1GQI is a Single protein structure of sequence from Cellvibrio japonicus with , and as ligands. Active as Alpha-glucuronidase, with EC number 3.2.1.139 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The structural basis for catalysis and specificity of the Pseudomonas cellulosa alpha-glucuronidase, GlcA67A., Nurizzo D, Nagy T, Gilbert HJ, Davies GJ, Structure. 2002 Apr;10(4):547-56. PMID:11937059
Page seeded by OCA on Sun Feb 3 09:41:55 2008
