1gzb
From Proteopedia
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PEROXIDASE
Overview
In the presence of ammonium sulfate the absorption spectra of a peroxidase, from the fungus Arthromyces ramosus (ARP) showed that the low-spin, component increased as the pH increased from 6.0 to 9.0, whereas in its, absence ARP remained in the high-spin state in the pH range investigated., The crystal structure of ARP at pH 4.5 in the presence of ammonium sulfate, at 1.8 A resolution showed that the electron density at the 6th position, of the heme iron seen at pH 7.5 had disappeared and that the iron atom, deviated markedly from the heme plane. These observations strongly suggest, that under physiological conditions the heme of ARP is in the, pentacoordinated high-spin state and that at a high pH the heme iron is, able to bind ammonia, forming the low-spin state. The location of the, water molecule at the distal side of the heme in peroxidases is also, discussed.
About this Structure
1GZB is a Single protein structure of sequence from Eukaryota with and as ligands. Active as Peroxidase, with EC number 1.11.1.7 Known structural/functional Sites: , and . Full crystallographic information is available from OCA.
Reference
Pentacoordination of the heme iron of Arthromyces ramosus peroxidase shown by a 1.8 A resolution crystallographic study at pH 4.5., Kunishima N, Amada F, Fukuyama K, Kawamoto M, Matsunaga T, Matsubara H, FEBS Lett. 1996 Jan 15;378(3):291-4. PMID:8557120
Page seeded by OCA on Sun Feb 3 09:44:18 2008
Categories: Eukaryota | Peroxidase | Single protein | Amada, F. | Fukuyama, K. | Kunishima, N. | CA | HEM | Glycoprotein | Heme | Oxidoreductase
