1hmh
From Proteopedia
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THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME
Overview
The hammerhead ribozyme is a small catalytic RNA motif made up of three, base-paired stems and a core of highly conserved, non-complementary, nucleotides essential for catalysis. The X-ray crystallographic structure, of a hammerhead RNA-DNA ribozyme-inhibitor complex at 2.6 A resolution, reveals that the base-paired stems are A-form helices and that the core, has two structural domains. The first domain is formed by the sequence, 5'-CUGA following stem I and is a sharp turn identical to the uridine turn, of transfer RNA, whereas the second is a non-Watson-Crick three-base-pair, duplex with a divalent-ion binding site. The phosphodiester backbone of, the DNA inhibitor strand is splayed out at the phosphate 5' to the, cleavage site. The structure indicates that the ribozyme may destabilize a, substrate strand in order to facilitate twisting of the substrate to allow, cleavage of the scissile bond.
About this Structure
1HMH is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of a hammerhead ribozyme., Pley HW, Flaherty KM, McKay DB, Nature. 1994 Nov 3;372(6501):68-74. PMID:7969422
Page seeded by OCA on Sun Nov 25 02:47:06 2007
