2bii

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spinqualitylabelsall models 2bii, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CRYSTAL STRUCTURE OF NITRATE-REDUCING FRAGMENT OF ASSIMILATORY NITRATE REDUCTASE FROM PICHIA ANGUSTA

Overview

Nitrate assimilation in autotrophs provides most of the reduced nitrogen, on earth. In eukaryotes, reduction of nitrate to nitrite is catalyzed by, the molybdenum-containing NAD(P)H:nitrate reductase (NR; EC 1.7.1.1-3). In, addition to the molybdenum center, NR contains iron-heme and flavin, adenine dinucleotide as redox cofactors involved in an internal electron, transport chain from NAD(P)H to nitrate. Recombinant, catalytically active, Pichia angusta nitrate-reducing, molybdenum-containing fragment (NR-Mo), was expressed in P. pastoris and purified. Crystal structures for NR-Mo, were determined at 1.7 and 2.6 angstroms. These structures revealed a, unique slot for binding nitrate in the active site and identified key Arg, and Trp residues potentially involved in nitrate binding. ... [(full description)]

About this Structure

2BII is a [Single protein] structure of sequence from [Pichia angusta] with SO4, NA, MTV and GOL as [ligands]. Active as [[1]], with EC number [1.7.1.2]. Full crystallographic information is available from [OCA].

Reference

Structural basis of eukaryotic nitrate reduction: crystal structures of the nitrate reductase active site., Fischer K, Barbier GG, Hecht HJ, Mendel RR, Campbell WH, Schwarz G, Plant Cell. 2005 Apr;17(4):1167-79. Epub 2005 Mar 16. PMID:15772287

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