1i3z
From Proteopedia
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MURINE EAT2 SH2 DOMAIN IN COMPLEX WITH SLAM PHOSPHOPEPTIDE
Overview
The T and natural killer (NK) cell-specific gene SAP (SH2D1A) encodes a, 'free SH2 domain' that binds a specific tyrosine motif in the cytoplasmic, tail of SLAM (CD150) and related cell surface proteins. Mutations in, SH2D1A cause the X-linked lymphoproliferative disease, a primary, immunodeficiency. Here we report that a second gene encoding a free SH2, domain, EAT-2, is expressed in macrophages and B lympho cytes. The EAT-2, structure in complex with a phosphotyrosine peptide containing a sequence, motif with Tyr281 of the cytoplasmic tail of CD150 is very similar to the, structure of SH2D1A complexed with the same peptide. This explains the, high affinity of EAT-2 for the pTyr motif in the cytoplasmic tail of CD150, but, unlike SH2D1A, EAT-2 does not bind to non-phosphorylated CD150. EAT-2, binds to the phosphorylated receptors CD84, CD150, CD229 and CD244, and, acts as a natural inhibitor, which interferes with the recruitment of the, tyrosine phosphatase SHP-2. We conclude that EAT-2 plays a role in, controlling signal transduction through at least four receptors expressed, on the surface of professional antigen-presenting cells.
About this Structure
1I3Z is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structural basis for the interaction of the free SH2 domain EAT-2 with SLAM receptors in hematopoietic cells., Morra M, Lu J, Poy F, Martin M, Sayos J, Calpe S, Gullo C, Howie D, Rietdijk S, Thompson A, Coyle AJ, Denny C, Yaffe MB, Engel P, Eck MJ, Terhorst C, EMBO J. 2001 Nov 1;20(21):5840-52. PMID:11689425
Page seeded by OCA on Tue Nov 20 17:02:15 2007
