1idp
From Proteopedia
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Crystal structure of scytalone dehydratase F162A mutant in the unligated state
Overview
Scytalone dehydratase variant F162A, in which Phe162 in the C-terminal, region was replaced with alanine, was crystallized with polyethylene, glycol 4000. Because the crystal was radiation-sensitive, the diffraction, data were collected at cryogenic temperatures. The crystal belonged to, monoclinic space group P2(1), with unit-cell parameters a = 72.64, b =, 61.30, c = 72.62 A, beta = 120.02 degrees at 37 K. The calculated V(M), value was acceptable when a trimer of the mutant enzyme occupied a, crystallographic asymmetric unit. The resolution limit was extended to, 1.45 A at BL41XU of SPring-8 at 37 K.
About this Structure
1IDP is a Single protein structure of sequence from Magnaporthe grisea. Active as Scytalone dehydratase, with EC number 4.2.1.94 Full crystallographic information is available from OCA.
Reference
Crystallization of scytalone dehydratase F162A mutant in the unligated state and a preliminary X-ray diffraction study at 37 K., Motoyama T, Nakasako M, Yamaguchi I, Acta Crystallogr D Biol Crystallogr. 2002 Jan;58(Pt 1):148-50. Epub 2001, Dec 21. PMID:11752795
Page seeded by OCA on Tue Nov 20 17:19:12 2007
