1ihv
From Proteopedia
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SOLUTION STRUCTURE OF THE DNA BINDING DOMAIN OF HIV-1 INTEGRASE, NMR, MINIMIZED AVERAGE STRUCTURE
Overview
The solution structure of the DNA binding domain of HIV-1 integrase, (residues 220-270) has been determined by multidimensional NMR, spectroscopy. The protein is a dimer in solution, and each subunit is, composed of a five-stranded beta-barrel with a topology very similar to, that of the SH3 domain. The dimer is formed by a stacked beta-interface, comprising strands 2, 3, and 4, with the two triple-stranded antiparallel, beta-sheets, one from each subunit, oriented antiparallel to each other., One surface of the dimer, bounded by the loop between strands beta 1 and, beta 2, forms a saddle-shaped groove with dimensions of approximately 24 x, 23 x 12 A in cross section. Lys264, which has been shown from mutational, data to be involved in DNA binding, protrudes from this surface, implicating the saddle-shaped groove as the potential DNA binding site.
About this Structure
1IHV is a Single protein structure of sequence from Human immunodeficiency virus. Full crystallographic information is available from OCA.
Reference
Solution structure of the DNA binding domain of HIV-1 integrase., Lodi PJ, Ernst JA, Kuszewski J, Hickman AB, Engelman A, Craigie R, Clore GM, Gronenborn AM, Biochemistry. 1995 Aug 8;34(31):9826-33. PMID:7632683
Page seeded by OCA on Thu Nov 8 14:10:00 2007
