1ilf
From Proteopedia
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NMR STRUCTURE OF APO CBFB
Overview
Runx proteins constitute a family of mammalian transcription factors that, interact with DNA through their evolutionarily conserved Runt domain., CBFbeta, alternatively denoted PEBP2beta, is the non-DNA-binding, heterodimer partner and acts to enhance the DNA binding affinity of Runx, proteins. Runx proteins and CBFbeta are associated with a variety of, biological functions and human diseases; they are, for example, together, the most frequent targets for chromosomal rearrangements in acute human, leukemias. We have determined the solution structure and characterized the, backbone dynamics of C-terminally truncated fragments containing residues, 1-141 of CBFbeta. The present apo-CBFbeta structure is very similar to, that seen in a Runt-CBFbeta complex. An evaluation of backbone (15)N NMR, relaxation parameters shows that CBFbeta is a rigid molecule with high, order parameters throughout the backbone; the only regions displaying, significant dynamics are a long loop and the C-terminal alpha-helix. A few, residues display relaxation behavior indicating conformational exchange on, microsecond to millisecond time scales, but only one of these is located, at the Runt binding surface. Our structure and dynamics analysis of, CBFbeta therefore suggests that the protein binds to Runt without large, conformational changes or induced folding ("lock-and-key" interaction)., The apo-CBFbeta structure presented here exhibits several significant, differences with two other published NMR ensembles of very similar protein, fragments. The differences are located in four regions outside of the, central beta-barrel, whereas the beta-barrel itself is almost identical in, the three NMR structures. The comparison illustrates that independently, determined NMR structures may display rather large differences in backbone, conformation in regions that appear to be well-defined in each of the, calculated NMR ensembles.
About this Structure
1ILF is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structure and backbone dynamics of Apo-CBFbeta in solution., Wolf-Watz M, Grundstrom T, Hard T, Biochemistry. 2001 Sep 25;40(38):11423-32. PMID:11560490
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