1iw4

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Solution structure of ascidian trypsin inhibitor

Overview

The three-dimensional solution structure of ascidian trypsin inhibitor, (ATI), a 55 amino acid residue protein with four disulfide bridges, was, determined by means of two-dimensional nuclear magnetic resonance (2D NMR), spectroscopy. The resulting structure of ATI was characterized by an, alpha-helical conformation in residues 35-42 and a three-stranded, antiparallel beta-sheet in residues 22-26, 29-32, and 48-50. The presence, of an alpha-helical conformation was predicted from the consensus, sequences of the cystine-stabilized alpha-helical (CSH) motif, which is, characterized by an alpha-helix structure in the Cys-X(1)-X(2)-X(3)-Cys, portion (corresponding to residues 37-41), linking to the Cys-X-Cys, portion (corresponding to residues 12-14) folded in an extended structure., The secondary structure and the overall folding of the main chain of ATI, were very similar to those of the Kazal-type inhibitors, such as Japanese, quail ovomucoid third domain (OMJPQ3) and leech-derived tryptase inhibitor, form C (LDTI-C), although ATI does not show extensive sequence homology to, these inhibitors except for a few amino acid residues and six of eight, half-cystines. On the basis of these findings, we realign the amino acid, sequences of representative Kazal-type inhibitors including ATI and, discuss the unique structure of ATI with four disulfide bridges.

About this Structure

1IW4 is a Single protein structure of sequence from Halocynthia roretzi. Full crystallographic information is available from OCA.

Reference

Solution structure of ascidian trypsin inhibitor determined by nuclear magnetic resonance spectroscopy., Hemmi H, Yoshida T, Kumazaki T, Nemoto N, Hasegawa J, Nishioka F, Kyogoku Y, Yokosawa H, Kobayashi Y, Biochemistry. 2002 Aug 27;41(34):10657-64. PMID:12186551

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