2brt
From Proteopedia
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ANTHOCYANIDIN SYNTHASE FROM ARABIDOPSIS THALIANA COMPLEXED WITH NARINGENIN
Overview
During the biosynthesis of the tricyclic flavonoid natural products in, plants, oxidative modifications to the central C-ring are catalysed by, Fe(ii) and 2-oxoglutarate dependent oxygenases. The reactions catalysed by, three of these enzymes; flavone synthase I, flavonol synthase and, anthocyanidin synthase (ANS), are formally desaturations. In comparison, flavanone 3beta-hydroxylase catalyses hydroxylation at the C-3 pro-R, position of 2S-naringenin. Incubation of ANS with the unnatural substrate, (+/-)-naringenin results in predominantly C-3 hydroxylation to give, cis-dihydrokaempferol as the major product; trans-dihydrokaempferol and, the desaturation product, apigenin are also observed. Labelling studies, have demonstrated that some of the formal desaturation reactions catalysed, by ... [(full description)]
About this Structure
2BRT is a [Single protein] structure of sequence from [Arabidopsis thaliana] with FE2, AKG and NAR as [ligands]. Active as [[1]], with EC number [1.14.11.19]. Full crystallographic information is available from [OCA].
Reference
Structural and mechanistic studies on anthocyanidin synthase catalysed oxidation of flavanone substrates: the effect of C-2 stereochemistry on product selectivity and mechanism., Welford RW, Clifton IJ, Turnbull JJ, Wilson SC, Schofield CJ, Org Biomol Chem. 2005 Sep 7;3(17):3117-26. Epub 2005 Aug 1. PMID:16106293
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