1jnt
From Proteopedia
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NMR Structure of the E. coli Peptidyl-Prolyl cis/trans-Isomerase Parvulin 10
Overview
E. coli Par10 is a peptidyl-prolyl cis/trans isomerase (PPIase) from, Escherichia coli catalyzing the isomerization of Xaa-Pro bonds in, oligopeptides with a broad substrate specificity. The structure of E. coli, Par10 has been determined by multidimensional solution-state NMR, spectroscopy based on 1207 conformational constraints (1067 NOE-derived, distances, 42 vicinal coupling-constant restraints, 30 hydrogen-bond, restraints, and 68 phi/psi restraints derived from the Chemical Shift, Index). Simulated-annealing calculations with the program ARIA and, subsequent refinement with XPLOR yielded a set of 18 convergent structures, with an average backbone RMSD from mean atomic coordinates of 0.50 A, within the well-defined secondary structure elements. E. coli Par10 is the, smallest known PPIase so far, with a high catalytic efficiency comparable, to that of FKBPs and cyclophilins. The secondary structure of E. coli, Par10 consists of four helical regions and a four-stranded antiparallel, beta-sheet. The N terminus forms a beta-strand, followed by a large, stretch comprising three alpha-helices. A loop region containing a short, beta-strand separates these helices from a fourth alpha-helix. The C, terminus consists of two more beta-strands completing the four-stranded, anti-parallel beta-sheet with strand order 2143. Interestingly, the third, beta-strand includes a Gly-Pro cis peptide bond. The curved beta-strand, forms a hydrophobic binding pocket together with alpha-helix 4, which also, contains a number of highly conserved residues. The three-dimensional, structure of Par10 closely resembles that of the human proteins hPin1 and, hPar14 and the plant protein Pin1At, belonging to the same family of, highly homologous proteins.
About this Structure
1JNT is a Single protein structure of sequence from Escherichia coli. Active as Peptidylprolyl isomerase, with EC number 5.2.1.8 Full crystallographic information is available from OCA.
Reference
Solution structure of Escherichia coli Par10: The prototypic member of the Parvulin family of peptidyl-prolyl cis/trans isomerases., Kuhlewein A, Voll G, Hernandez Alvarez B, Kessler H, Fischer G, Rahfeld JU, Gemmecker G, Protein Sci. 2004 Sep;13(9):2378-87. PMID:15322281
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