1jui
From Proteopedia
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CONCANAVALIN A-CARBOHYDRATE MIMICKING 10-MER PEPTIDE COMPLEX
Overview
The structures of concanavalin A (ConA) in complex with two, carbohydrate-mimicking peptides, 10-mer (MYWYPYASGS) and 15-mer, (RVWYPYGSYLTASGS) have been determined at 2.75 A resolution. In both, crystal structures four independent peptide molecules bind to each of the, crystallographically independent subunits of ConA tetramer. The peptides, exhibit small but significant variability in conformations and, interactions while binding to ConA. The crystal structure of another, similar peptide, 12-mer (DVFYPYPYASGS), in complex with ConA has been, determined (Jain, D., K. J. Kaur, B. Sundaravadivel, and D. M. Salunke., 2000. Structural and functional consequences of peptide-carbohydrate, mimicry. J. Biol. Chem. 275:16098-16102). Comparison of the three, complexes shows that the peptides bind to ConA at a common binding site, using different contacting residues and interactions depending on their, sequence and the local environment at the binding site. The binding is, also optimized by corresponding plasticity of the peptide binding site on, ConA. The diversity in conformation and interactions observed here are in, agreement with the structural leeway concerning plasticity of specific, molecular recognition in biological processes. The adaptability of, peptide-ConA interactions may also be correlated with the, carbohydrate-mimicking property of these peptides.
About this Structure
1JUI is a Single protein structure of sequence from Canavalia ensiformis with MN and CA as ligands. Full crystallographic information is available from OCA.
Reference
Plasticity in protein-peptide recognition: crystal structures of two different peptides bound to concanavalin A., Jain D, Kaur KJ, Salunke DM, Biophys J. 2001 Jun;80(6):2912-21. PMID:11371463
Page seeded by OCA on Tue Nov 20 18:35:23 2007
Categories: Canavalia ensiformis | Single protein | Jain, D. | Kaur, K.J. | Salunke, D.M. | CA | MN | Lectin
