1k1e
From Proteopedia
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Structure Of the cobalt-bound form of the deoxy-D-mannose-octulosonate 8-phosphate phosphatase (YrbI) From Haemophilus Influenzae (HI1679)
Overview
The crystal structure of the YrbI protein from Haemophilus influenzae, (HI1679) was determined at a 1.67-A resolution. The function of the, protein had not been assigned previously, and it is annotated as, hypothetical in sequence databases. The protein exhibits the, alpha/beta-hydrolase fold (also termed the Rossmann fold) and resembles, most closely the fold of the L-2-haloacid dehalogenase (HAD) superfamily., Following this observation, a detailed sequence analysis revealed remote, homology to two members of the HAD superfamily, the P-domain of Ca(2+), ATPase and phosphoserine phosphatase. The 19-kDa chains of HI1679 form a, tetramer both in solution and in the crystalline form. The four monomers, are arranged in a ring such that four beta-hairpin loops, each inserted, after the first beta-strand of the core alpha/beta-fold, form an, eight-stranded barrel at the center of the assembly. Four active sites are, located at the subunit interfaces. Each active site is occupied by a, cobalt ion, a metal used for crystallization. The cobalt is octahedrally, coordinated to two aspartate side-chains, a backbone oxygen, and three, solvent molecules, indicating that the physiological metal may be, magnesium. HI1679 hydrolyzes a number of phosphates, including, 6-phosphogluconate and phosphotyrosine, suggesting that it functions as a, phosphatase in vivo. The physiological substrate is yet to be identified;, however the location of the gene on the yrb operon suggests involvement in, sugar metabolism.
About this Structure
1K1E is a Single protein structure of sequence from Haemophilus influenzae with CO, HG, SO4, MES and GOL as ligands. Full crystallographic information is available from OCA.
Reference
From structure to function: YrbI from Haemophilus influenzae (HI1679) is a phosphatase., Parsons JF, Lim K, Tempczyk A, Krajewski W, Eisenstein E, Herzberg O, Proteins. 2002 Mar 1;46(4):393-404. PMID:11835514
Page seeded by OCA on Tue Nov 20 18:46:16 2007
