1k8z
From Proteopedia
|
CRYSTAL STRUCTURE OF THE TRYPTOPHAN SYNTHASE BETA-SER178PRO MUTANT COMPLEXED WITH N-[1H-INDOL-3-YL-ACETYL]GLYCINE ACID
Overview
The catalytic activity of the pyridoxal 5'-phosphate-dependent tryptophan, synthase alpha(2)beta(2) complex is allosterically regulated. The hydrogen, bond between the helix betaH6 residue betaSer(178) and the loop alphaL6, residue Gly(181) was shown to be critical in ligand-induced intersubunit, signaling, with the alpha-beta communication being completely lost in the, mutant betaSer(178) --> Pro (Marabotti, A., De Biase, D., Tramonti, A., Bettati, S., and Mozzarelli, A. (2001) J. Biol. Chem. 276, 17747-17753)., The structural basis of the impaired allosteric regulation was, investigated by determining the crystal structures of the mutant, betaSer(178) --> Pro in the absence and presence of the alpha-subunit, ligands indole-3-acetylglycine and glycerol 3-phosphate. The mutation, causes local and distant conformational changes especially in the, beta-subunit. The ligand-free structure exhibits larger differences at the, N-terminal part of helix betaH6, whereas the enzyme ligand complexes show, differences at the C-terminal side. In contrast to the wild-type enzyme, loop alphaL6 remains in an open conformation even in the presence of, alpha-ligands. This effects the equilibrium between active and inactive, conformations of the alpha-active site, altering k(cat) and K(m), and, forms the structural basis for the missing allosteric communication, between the alpha- and beta-subunits.
About this Structure
1K8Z is a Protein complex structure of sequences from Salmonella typhimurium with NA, IAG and PLP as ligands. Active as Tryptophan synthase, with EC number 4.2.1.20 Full crystallographic information is available from OCA.
Reference
Crystal structure of the beta Ser178--> Pro mutant of tryptophan synthase. A "knock-out" allosteric enzyme., Weyand M, Schlichting I, Herde P, Marabotti A, Mozzarelli A, J Biol Chem. 2002 Mar 22;277(12):10653-60. Epub 2001 Dec 26. PMID:11756454
Page seeded by OCA on Tue Nov 20 18:57:57 2007
