1kq4

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spinqualitylabelsall models 1kq4, resolution 2.25Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal Structure of Thy1-Complementing Protein (TM0449) from Thermotoga maritima at 2.25 A Resolution

Overview

Structural genomics is emerging as a principal approach to define protein, structure-function relationships. To apply this approach on a genomic, scale, novel methods and technologies must be developed to determine large, numbers of structures. We describe the design and implementation of a, high-throughput structural genomics pipeline and its application to the, proteome of the thermophilic bacterium Thermotoga maritima. By using this, pipeline, we successfully cloned and attempted expression of 1,376 of the, predicted 1,877 genes (73%) and have identified crystallization conditions, for 432 proteins, comprising 23% of the T. maritima proteome., Representative structures from TM0423 glycerol dehydrogenase and TM0449, thymidylate synthase-complementing protein are presented as examples of, final outputs from the pipeline.

About this Structure

1KQ4 is a Single protein structure of sequence from Thermotoga maritima with FAD as ligand. Full crystallographic information is available from OCA.

Reference

Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline., Lesley SA, Kuhn P, Godzik A, Deacon AM, Mathews I, Kreusch A, Spraggon G, Klock HE, McMullan D, Shin T, Vincent J, Robb A, Brinen LS, Miller MD, McPhillips TM, Miller MA, Scheibe D, Canaves JM, Guda C, Jaroszewski L, Selby TL, Elsliger MA, Wooley J, Taylor SS, Hodgson KO, Wilson IA, Schultz PG, Stevens RC, Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11664-9. Epub 2002 Aug 22. PMID:12193646

Page seeded by OCA on Tue Nov 20 19:36:57 2007