1krm
From Proteopedia
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Crystal structure of bovine adenosine deaminase complexed with 6-hydroxyl-1,6-dihydropurine riboside
Overview
The crystal structure of adenosine deaminase (ADA) from bovine intestine, complexed with a transition-state analogue, 6-hydroxy-1,6-dihydropurine, riboside (HDPR), was solved at 2.5 A resolution by the, molecular-replacement method using a homology model based on the crystal, structure of mouse ADA. The final refinement converged to a, crystallographic R factor of 20.7%. The C(alpha) backbone of bovine ADA is, mostly superimposable on that of mouse ADA, although mouse ADA itself did, not lead to a solution by molecular replacement. HDPR tightly interacts, with ADA by means of six hydrogen bonds and is entirely enclosed within, the active site. The lid of the envelope consists of two components: one, contains two leucine residues, Leu55 and Leu59, and the other contains the, backbone atoms Asp182 and Glu183. The C(delta) atoms of the two leucine, residues are 3.5 A from the respective N atoms of the backbone. A weak, interaction, similar to CH-pi binding, might make it possible to open the, lid. Taking account of the movement and observation of this structural, feature, the aim is to design novel ADA inhibitors.
About this Structure
1KRM is a Single protein structure of sequence from Bos taurus with ZN and PRH as ligands. Active as Adenosine deaminase, with EC number 3.5.4.4 Full crystallographic information is available from OCA.
Reference
Structure of bovine adenosine deaminase complexed with 6-hydroxy-1,6-dihydropurine riboside., Kinoshita T, Nishio N, Nakanishi I, Sato A, Fujii T, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):299-303. Epub 2003, Jan 23. PMID:12554940
Page seeded by OCA on Tue Nov 20 19:45:24 2007
