1l3d
From Proteopedia
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Low Resolution Crystal Structure of a Viral RNA Pseudoknot
Overview
Many pathogenic viruses use programmed -1 ribosomal frameshifting to, regulate translation of their structural and enzymatic proteins from, polycistronic mRNAs. Frameshifting is commonly stimulated by a pseudoknot, located downstream from a slippery sequence, the latter positioned at the, ribosomal A and P sites. We report here the structures of two crystal, forms of the frameshifting RNA pseudoknot from beet western yellow virus, at resolutions of 1.25 and 2.85 A. Because of the very high resolution of, 1.25 A, ten mono- and divalent metal ions per asymmetric unit could be, identified, giving insight into potential roles of metal ions in, stabilizing the pseudoknot. A magnesium ion located at the junction of the, two pseudoknot stems appears to play a crucial role in stabilizing the, structure. Because the two crystal forms exhibit mostly unrelated packing, interactions and local crystallographic disorder in the high-resolution, form was resolvable, the two structures offer the most detailed view yet, of the conformational preference and flexibility of an RNA pseudoknot.
About this Structure
1L3D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Metal ions and flexibility in a viral RNA pseudoknot at atomic resolution., Egli M, Minasov G, Su L, Rich A, Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4302-7. Epub 2002 Mar 19. PMID:11904368
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Categories: Protein complex | Egli, M. | Minasov, G. | Rich, A. | Su, L. | Flexibility | Frameshifting | Pseudoknot | Viral rna
