1lbg
From Proteopedia
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LACTOSE OPERON REPRESSOR BOUND TO 21-BASE PAIR SYMMETRIC OPERATOR DNA, ALPHA CARBONS ONLY
Overview
The lac operon of Escherichia coli is the paradigm for gene regulation., Its key component is the lac repressor, a product of the lacI gene. The, three-dimensional structures of the intact lac repressor, the lac, repressor bound to the gratuitous inducer, isopropyl-beta-D-1-thiogalactoside (IPTG) and the lac repressor complexed, with a 21-base pair symmetric operator DNA have been determined. These, three structures show the conformation of the molecule in both the induced, and repressed states and provide a framework for understanding a wealth of, biochemical and genetic information. The DNA sequence of the lac operon, has three lac repressor recognition sites in a stretch of 500 base pairs., The crystallographic structure of the complex with DNA suggests that the, tetrameric repressor functions synergistically with catabolite gene, activator protein (CAP) and participates in the quaternary formation of, repression loops in which one tetrameric repressor interacts, simultaneously with two sites on the genomic DNA.
About this Structure
1LBG is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of the lactose operon repressor and its complexes with DNA and inducer., Lewis M, Chang G, Horton NC, Kercher MA, Pace HC, Schumacher MA, Brennan RG, Lu P, Science. 1996 Mar 1;271(5253):1247-54. PMID:8638105
Page seeded by OCA on Tue Nov 20 20:26:51 2007
