1lf6
From Proteopedia
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CRYSTAL STRUCTURE OF BACTERIAL GLUCOAMYLASE
Overview
The first crystal structures of a two-domain, prokaryotic glucoamylase, were determined to high resolution from the clostridial species, Thermoanaerobacterium thermosaccharolyticum with and without acarbose. The, N-terminal domain has 18 antiparallel strands arranged in beta-sheets of a, super-beta-sandwich. The C-terminal domain is an (alpha/alpha)(6) barrel, lacking the peripheral subdomain of eukaryotic glucoamylases. Interdomain, contacts are common to all prokaryotic Family GH15 proteins. Domains, similar to those of prokaryotic glucoamylases in maltose phosphorylases, (Family GH65) and glycoaminoglycan lyases (Family PL8) suggest evolution, from a common ancestor. Eukaryotic glucoamylases may have evolved from, prokaryotic glucoamylases by the substitution of the N-terminal domain, with the peripheral subdomain and by the addition of a starch-binding, domain.
About this Structure
1LF6 is a Single protein structure of sequence from Thermoanaerobacterium thermosaccharolyticum with SO4 as ligand. Active as Glucan 1,4-alpha-glucosidase, with EC number 3.2.1.3 Full crystallographic information is available from OCA.
Reference
Crystal structure and evolution of a prokaryotic glucoamylase., Aleshin AE, Feng PH, Honzatko RB, Reilly PJ, J Mol Biol. 2003 Mar 14;327(1):61-73. PMID:12614608
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