1lmc
From Proteopedia
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THE CRYSTAL STRUCTURE OF A COMPLEX BETWEEN BULGECIN, A BACTERIAL METABOLITE, AND LYSOZYME FROM THE RAINBOW TROUT
Overview
Bulgecin, a sulfonated glycopeptide produced by Pseudomonas acidophila and, Pseudomonas mesoacidophila, induces bulge formation and enhances lysis of, bacterial cell walls when used in combination with beta-lactam, antibiotics. The compound does not itself exhibit any antibacterial, activity, but has been shown to inhibit a soluble lytic transglycosylase, (SLT70) from Escherichia coli which has a lysozyme-like domain. Recently, the crystal structure of an SLT-bulgecin complex has been determined to, 3.5 A resolution. We report here the crystal structure of a complex, between lysozyme from the rainbow trout (RBTL) and bulgecin A at 2.0 A, resolution. As for the SLT-bulgecin complex, bulgecin is bound with the, glycosaminyl moiety in subsite C and the proline residue in site D of the, active-site cleft of RBTL, where it makes hydrogen-bonding interactions, with the catalytic residues. The taurine moiety is bound to the left side, of subsites E and F in the lower part of the active-site cleft. From the, observed position of the bulgecin molecule, it seems reasonable that it is, an inhibitor of rainbow trout lysozyme. The lysozymes may, in general, be, a target for the design of a novel type of antibiotics distinct from the, beta-lactams which are insensitive to the muramidases.
About this Structure
1LMC is a Single protein structure of sequence from Oncorhynchus mykiss with BUL as ligand. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.
Reference
Structure of a complex between bulgecin, a bacterial metabolite, and lysozyme from the rainbow trout., Karlsen S, Hough E, Acta Crystallogr D Biol Crystallogr. 1996 Jan 1;52(Pt 1):115-23. PMID:15299732
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