1m3e
From Proteopedia
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Succinyl-COA:3-ketoacid COA transferase from pig heart (selenomethionine)
Overview
Ketoacidosis affects patients who are deficient in the enzyme activity of, succinyl-CoA:3-ketoacid CoA transferase (SCOT), since SCOT catalyses the, activation of acetoacetate in the metabolism of ketone bodies. Thus far, structure/function analysis of the mammalian enzyme has been predicted, based on the three-dimensional structure of a CoA transferase determined, from an anaerobic bacterium that utilizes its enzyme for glutamate, fermentation. To better interpret clinical data, we have determined the, structure of a mammalian CoA transferase from pig heart by X-ray, crystallography to 2.5 A resolution. Instrumental to the structure, determination were selenomethionine substitution and the use of argon, during purification and crystallization. Although pig heart SCOT adopts an, alpha/beta protein fold, resembling the overall fold of the bacterial CoA, transferase, several loops near the active site of pig heart SCOT follow, different paths than the corresponding loops in the bacterial enzyme, accounting for differences in substrate specificities. Two missense, mutations found associated with SCOT of ketoacidosis patients were mapped, to a location in the structure that might disrupt the stabilization of the, amino-terminal strand and thereby interfere with the proper folding of the, protein into a functional enzyme.
About this Structure
1M3E is a Single protein structure of sequence from Sus scrofa. Active as 3-oxoacid CoA-transferase, with EC number 2.8.3.5 Full crystallographic information is available from OCA.
Reference
Structure of the mammalian CoA transferase from pig heart., Bateman KS, Brownie ER, Wolodko WT, Fraser ME, Biochemistry. 2002 Dec 10;41(49):14455-62. PMID:12463743
Page seeded by OCA on Tue Nov 20 21:07:01 2007
